Service Details: Single Nuclei Gene Expression
What is included?
Sample Preparation
Sample QC
Library Preparation
Sequencing
Cell Ranger Pipeline and Loupe Browser
Data Delivery
Technical Specifications
Library Preparation Kit: Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Dual Index)
Sequencing Platform: Illumina NovaSeq 6000
Read Type: Paired-end
Sequencing Depth: 20,000 read pairs per Nuclei for 3' gene expression
Deliverables
Raw data file (.fastq)
10X Loupe Browser files (.cloupe)
10X Cell Ranger WebSummary (.html)
Summary QC Report (.pdf)
Turnaround Time
4 weeks for a batch of up to 16 samples
Sample Requirements
For detailed information about the sample requirements for our Single Nuclei Gene Expression service, please consult our sample requirements page.
We currently offer Single Nuclei Gene expression from snap frozen liver tissue including fatty and fibrotic tissue. Please, contact us if you are interested in this service for other tissues, such as brain, heart, or kidney.
Why Choose Our Single Nuclei Gene Expression Services?
Why Choose Single Nuclei Gene Expression?
Applications of Single Nuclei Gene Expression?
Service Workflow: Single Nuclei Gene Expression
