DNA Extraction (if applicable)
Initial DNA QC
Primer design
Sample PCR
Sanger Sequencing
QC review
Analysis (optional)
Sanger Sequencing Kit: Big Dye Direct Cycle Sequencing Kit and BigDye XTerminator™ Purification Kit
Sequencing Platform: SeqStudio Genetic Analyser
Read Length (CRL): Short ≥350bp , Medium ≥ 500bp, Long ≥800bp
Initial DNA QC Report (.pdf)
Sanger Sequencing QC Report (.pdf)
Electropherogram (.ab1)
Sequence text file (.fasta)
Optional Analysis Report (.pdf)
2-3 weeks for a batch of up to 24 samples (with a single primer set)
For detailed information about the sample requirements for our Sanger Sequencing service, please consult our sample requirements page.