Sanger Sequencing

Service Details: Sanger Sequencing

What is included?

DNA Extraction (if applicable)
Initial DNA QC
Primer design
Sample PCR
Sanger Sequencing
QC review
Analysis (optional)

Technical Specifications

Sanger Sequencing Kit: Big Dye Direct Cycle Sequencing Kit and BigDye XTerminator™ Purification Kit

Sequencing Platform: SeqStudio Genetic Analyser

Read Length (CRL): Short ≥350bp , Medium ≥ 500bp, Long ≥800bp

Deliverables

Initial DNA QC Report (.pdf)
Sanger Sequencing QC Report (.pdf)
Electropherogram (.ab1)
Sequence text file (.fasta)
Optional Analysis Report (.pdf)

Turnaround Time

2-3 weeks for a batch of up to 24 samples (with a single primer set)

Sample Requirements

For detailed information about the sample requirements for our Sanger Sequencing service, please consult our sample requirements page.

*Turnaround times are indicative and may be subject to sample type and volume.

Why Choose Our Sanger Sequencing Services?

Why Choose Sanger Sequencing?

Applications of Sanger Sequencing?

Service Workflow: Sanger Sequencing

Service Workflow: Sanger Sequencing
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