Biopharma / Research Service
Total RNA Sequencing
RNA sequencing, or RNA-Seq, is a high-throughput genetic analysis technique that affords a deeper understanding of the dynamics of gene expression. Unlike traditional DNA-focused methods, RNA sequencing quantifies the expression of all the transcribed RNA molecules in a single comprehensive assay. This approach provides invaluable insights into which genes are actively transcribed, their abundance, and how their expression changes under different conditions or in response to specific treatments.
Service Details: Total RNA Sequencing
What is included?
RNA Extraction (if applicable)
Initial RNA QC
Library Preparation (Ribo and Globin depletion)
Sequencing
Alignment
Gene Expression
Data Delivery
Technical Specifications
Library Preparation Kit: Illumina TruSeq Stranded Total RNA Library Prep Globin
Sequencing Platform: Illumina NovaSeq 6000
Read Type: Paired-end
Read Length: 75bp
Sequencing Depth: 50M paired reads, 100M total reads (alternative coverage options available upon request)
Deliverables
Initial RNA QC Report (.pdf)
Raw data file (.fastq)
Alignment file (.bam)
Gene count file (.genecounts.tsv)
Sequencing QC Metrics File (.pdf)
Turnaround Time
1-2 weeks for a batch of up to 96 samples
Sample Requirements
For detailed information about the sample requirements for our Total RNA Sequencing service, please consult our sample requirements page.
Why Choose Our Total RNA Sequencing Services?
Why Choose Total RNA Sequencing?
Applications of Total RNA Sequencing?
Service Workflow: Total RNA Sequencing
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