Biopharma / Research Service
Single Cell Gene Expression, also known as single-cell RNA sequencing (scRNA-Seq), is a cutting-edge analysis technique that allows for the precise quantification of gene expression within individual cells. By capturing gene expression data from thousands of single cells simultaneously, this method enables the exploration of cellular heterogeneity, uncovering variations in gene expression that may be obscured in bulk RNA sequencing analyses. Through the examination of the unique expression profiles of individual cells, this method facilitates a more profound comprehension of cellular behaviour and disease mechanisms.
Sample Preparation
Sample QC
Library Preparation
Sequencing
Cell Ranger Pipeline and Loupe Browser
Data Delivery
Library Preparation Kit: Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Dual Index)
Sequencing Platform: Illumina NovaSeq 6000
Read Type: Paired-end
Sequencing Depth: 20,000 read pairs per cell for 3' gene expression
Raw data file (.fastq)
10X Loupe Browser files (.cloupe)
10X Cell Ranger WebSummary (.html)
Summary QC Report (.pdf)
4 weeks for a batch of up to 16 samples
For detailed information about the sample requirements for our Single Cell Gene Expression service, please consult our sample requirements page.
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